KMID : 0380620110430050553
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Korean Journal of Food Science and Technology 2011 Volume.43 No. 5 p.553 ~ p.557
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Isolation and Purification of Garlic Specific Organic Compounds
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Oh Tae-Young
Kyung Kyu-Hang
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Abstract
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Garlic specific organic compounds were separated and purified using a recycling preparative high-performance liquid chromatography (HPLC) from blanched garlic cloves. Identification of the compounds involved comparing the previously reported HPLC retention times as well as other identification methods including 1H- and 13C-nuclear magnetic resonance and liquid chromatography-mass spectrometry. The yields of garlic specific organic compounds were 12.2, 42.5, 1.6, 1.2, and 4.8% on wet weight basis of garlic for alliin(S-allyl-L-cysteine sulfoxide), isoalliin(S-1-propenyl-L-cysteine sulfoxide), ¥ã-glutamyl-S-allylcysteine, ¥ã-glutamyl-S-1-propenylcysteine and ¥ã-glutamyl-phenylalanine, respectively. All the compounds, except for ¥ã-glutamylphenylalanine, contained sulfur.
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KEYWORD
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garlic, alliin, isoalliin, ¥ã-glutamyl-S-allylcysteine, ¥ã-glutamyl-S-1-propenylcysteine, ¥ã-glutamylphenylalanine
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